Polynucleotides for the detection of listeria species

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/11 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 15/31 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2613159

A system for the detection of a variety of Listeria species in a test sample is provided. The system comprises polynucleotide primers and probes for the amplification and detection of a portion of a Listeria ssrA gene. The primers and probes can be used in real time diagnostic assays for rapid detection of Listeria in a variety of situations. Kits comprising the primers and probes are also provided.

La présente invention a trait à un système pour la détection d'une variété de l'espèce Listeria dans un échantillon d'essai. Le système comporte des amorces et des sondes polynucléotidiques pour l'amplification et la détection d'une portion d'un gèneListeria ssrA. Les amorces et les sondes peuvent être utilisées dans des dosages de diagnostic en temps réel pour la détection rapide de Listeria dans diverses situations. L'invention a également trait à des trousses comportant les amorces et les sondes.

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