Polyoxyhydrocarbyl related products and methods for...

C - Chemistry – Metallurgy – 09 – B

Patent

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C09B 1/32 (2006.01) C09B 5/42 (2006.01) C09B 15/00 (2006.01) C09B 19/00 (2006.01) C09B 21/00 (2006.01) C09B 23/14 (2006.01) C09B 47/00 (2006.01) C09B 47/24 (2006.01) C09B 57/00 (2006.01) C09B 69/00 (2006.01) G01N 33/52 (2006.01) G01N 33/533 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2223418

Fluorescent dyes coupled to one or more polyoxyhydrocarbyl moieties which are free of aggregation and serum binding are provided. These dyes are suitable for applications such as fluorescence immunoassays, in vivo imaging and in vivo tumor therapy. Fluorescence immunoassays methods are provided which use fluorescent dyes which are free of aggregation and serum binding. Such immunoassay methods are thus, particularly useful for the assay of biological fluids, such as serum, plasma, whole blood and urine. The present invention is directed particularly to markers wherein the fluorophore is selected from the group consisting of aryl terminated polymethine dyes; quinoid dyes; 1,4-diaminoanthraquinone-2,3- dicarboxamides, indanthrene dyes; tetraaminoanthraquinones; azine dyes; (thio)pyriliamdyes and napthoquinone methides.

L'invention se rapporte à des colorants fluorescents qui ne peuvent s'agglomérer et se fixer au sérum. Ces colorants sont appropriés à des applications telles que des dosages immunologiques en fluorescence, l'imagerie in vivo et la thérapie in vivo des tumeurs. Ces méthodes de dosage immunologique en fluorescence mettent en oeuvre des colorants fluorescents qui ne peuvent s'agglomérer et se fixer au sérum. Ils sont donc particulièrement utiles dans le dosage de liquides biologiques tels que le sérum, le plasma, le sang entier et l'urine. La présente invention se rapporte également à des compositions comprenant un oligonucléotide lié à un composant de marqueur marqué de manière détectable comprenant une fraction fluorogène constituée d'un ligand macrocyclique, multidenté, pratiquement plat, coordonné à un atome central pouvant se coordonner à deux ligands axiaux et à deux fractions de polyoxyhydrocarbyle qui sont fixées comme ligands axiaux à l'atome central. La présente invention se rapporte également à l'hybridation de l'acide nucléique et à des procédés d'amplification dans lesquels ces compositions sont utilisées.

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