Polypeptide and protein products, and process for their...

Details Polypeptide and protein products, and process for their... Polypeptide and protein products, and process for their...

C - Chemistry, Metallurgy

12

N

530/15, 195/33.1

C12N 15/62 (2006.01) A61K 39/00 (2006.01) C07K 14/585 (2006.01) C07K 14/695 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1306207

Abstract A process for the production of a fusion protein comprising an active portion of a chloramphenicol acetyl- transferase (CAT) protein and a polypeptide. The fusion protein may be purified using CAT substrate affinity chromatography. The eucaryotic polypeptide may be calcitonin or a derivative thereof such as calcitonin-glycine. Other polypeptides described include enzymes such as chymosin, prochymosin and preprochymosin, hormones such as ACTH, insulins, and growth hormones and antigenic polypeptides such as foot and mouth disease virus antigenic polypeptide. The fusion protein may be cleaved at a site susceptible to selective enzymic or chemical cleavage to produce free polypeptide. The fusion protein may be used as an immunogen.

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