Polypeptide cleavage process

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/12 (2006.01) C07K 1/14 (2006.01) C07K 14/60 (2006.01) C07K 14/635 (2006.01) C07K 19/00 (2006.01) C12P 21/00 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2485737

A palladatepalladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladatepalladium promotor dissolved in a high- concentration acidic organic acid solvent.

Cette invention concerne un processus de clivage polypeptidique favorisé par palladate de palladium qui assure un clivage sélective du polypetide sur site de clivageCys-His. Cette technique consiste à solubiliser le polypetide dans un mélange réactif fait d'un promoteur palladate palladium dissous dans un solvant d'acide organique forte concentration.

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