Polypeptide expression method

Details Polypeptide expression method Polypeptide expression method

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/00 (2006.01) C07K 14/62 (2006.01) C12N 1/20 (2006.01) C12N 15/62 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1213537

22 ABSTRACT OF THE DISCLOSURE The stability of polypeptides, such as proinsulin, somatostatin and interferon, produced in microorganisms transformed by cloning vehicles containing a DNA sequence coding for the polypeptide, such that the polypeptide may be recovered as a product of the microorganism, is significantly improved by inserting into the cloning vehicle a DNA fragment which codes for tandemly-linked multiple copies of the polypeptide gene joined by linking sequences which code for easily-cleavable amino acid sequences. Stop and start codons are absent between the ends of the fragment, so that the whole fragment is expressed. High yields of the multiple copy (polymeric) product are obtained and may be cleaved at the contrived joins to provide single polypeptides. Novel plasmids for the specific example of human proinsulin are described.

453270

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