Polypeptide immobilization

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 80/00 (2006.01) C07C 323/58 (2006.01) C07F 9/40 (2006.01) C07F 9/655 (2006.01) C07K 1/04 (2006.01) C07K 5/037 (2006.01) C07K 14/435 (2006.01) C07K 17/00 (2006.01) C12N 15/12 (2006.01) C12N 15/66 (2006.01) C12Q 1/25 (2006.01) C12Q 1/37 (2006.01) C12Q 1/48 (2006.01) C40B 30/00 (2006.01) C40B 30/08 (2006.01) C40B 40/04 (2006.01) C40B 40/10 (2006.01) C40B 50/14 (2006.01) C40B 70/00 (2006.01) G01N 33/543 (2006.01) G01N 33/551 (2006.01) G01N 33/68 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2456698

A substrate comprises a surface, and a plurality of moieties, on at least a portion of the surface. The moieties are moieties of formula: Surf-L-Q-T, where -T comprises a reactant ligand, and Surf-designates where the moiety attaches to the surface. The substrate can be made into a protein chip by the reaction of a reactant ligand and a fusion polypeptide, where the fusion polypeptide includes a capture polypeptide moiety which corresponds to the reactant ligand.

Selon cette invention, un substrat comporte une surface et une pluralité de groupes caractéristiques sur au moins une partie de la surface. Ces groupes caractéristiques sont des groupes caractéristiques de formule: Surf-L-Q-T, dans laquelle -T comprend un ligand de réactif, et des parties désignées Surf-, dans lesquelles le groupe caractéristique se lie à la surface. Ce substrat peut être élaboré en une puce à protéine par réaction d'un ligand de réactif et d'un polypeptide de fusion, ledit polypeptide de fusion comportant un groupe caractéristique de polypeptide de capture qui correspond au ligand du réactif.

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