Polypeptide producing cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/79 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01) G01N 33/554 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2651478

The current invention describes a nucleic acid comprising in a 5' to 3' direction a) a first nucleic acid encoding a heterologous polypeptide without an in frame stop codon, b) a second nucleic acid beginning with a 5' splice donor site and terminated by a 3' splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal, and c) a nucleic acid encoding i) at least a fragment of a transmembrane domain, or ii) a signal peptide for a GPI-anchor.

L'invention concerne un acide nucléique contenant, dans une direction 5' vers 3', a) un premier acide nucléique codant un polypeptide hétérologue sans terminateur dans le cadre, b) un deuxième acide nucléique débutant par un site donneur d'épissure 5' et terminant par un site accepteur d'épissure 3', comportant un terminateur translationnel dans le cadre et un signal de polyadénylation, et c) un acide nucléique codant i) au moins un fragment d'un domaine transmembranaire ou ii) un peptide de signal pour une ancre GPI.

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