Polypeptides and biosynthetic pathways for the production of...

C - Chemistry – Metallurgy – 12 – P

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C12P 13/22 (2006.01) C12N 1/18 (2006.01)

Patent

CA 2606178

Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3- ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

L'invention concerne des procédés et des compositions destinés à être utilisés pour la production de monatine à partir de glucose, de tryptophane, d'acide indole-3-lactique, d'indole-3-pyruvate, et d'acide 2-hydroxy 2-(indol-3-ylméthyl)-4-céto glutarique. L'invention concerne également des procédés de production d'intermédiaires d'indole-3-pyruvate et d'acide 2-hydroxy 2-(indol-3-ylméthyl)-4-céto glutarique. Les compositions de l'invention comprennent des molécules d'acides nucléiques, des polypeptides, des structures chimiques, et des cellules. Les procédés de l'invention comprennent des procédés in vitro et in vivo, les procédés in vitro impliquant des réactions chimiques.

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