Polypeptides and biosynthetic pathways for the production of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/54 (2006.01) C07D 209/18 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12N 9/06 (2006.01) C12N 9/08 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12P 17/10 (2006.01)

Patent

CA 2483126

Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(iridol-3- ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for produding the indole-3-pyruvate and 2-hydroxy 2-(indol-3~ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

L'invention concerne des procédés et des compositions qui peuvent être utilisés pour fabriquer de la monatine à partir de glucose, tryptophan, acide indole-3-lactique, indole-3-pyruvate, et acide 2-hydroxy 2-(iridol-3-ylméthyl)-4-ceto glutarique. L'invention traite aussi de procédés de production de produits intermédiaires d'indole-3-pyruvate et d'acide 2-hydroxy 2-(indol-3-ylméthyl)-4-ceto glutarique. L'invention traite aussi de compositions qui comprennent des molécules d'acide nucléique, de polypeptides, de structures chimiques et de cellules. L'invention a aussi pour objet des procédés qui comprennent des procédés in vitro et in vivo et les procédés in vitro comprennent des réactions chimiques.

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