Polypeptides having a functional domain of interest and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C07K 19/00 (2006.01) C12N 15/10 (2006.01) C12N 15/12 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/554 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2217641

Novel polypeptides having functional domains of interest are described, along with DNA sequences that encode the same. A method of identifying these polypeptides by means of a sequence-independent (that is, independent of the primary sequence of the polypeptide sought), recognition unit-based functional screen is also disclosed. Various applications of the method and of the polypeptides identified are described, including their use in assay kits for drug discovery, modification, and refinement.

On décrit de nouveaux polypeptides qui présentent des domaines fonctionnels importants, ainsi que des séquences d'ADN qui les codent. On décrit aussi un procédé d'identification de ces polypeptides à l'aide d'un crible fonctionnel basé sur une unité de reconnaissance et indépendant de la séquence primaire du polypeptide recherché. On décrit enfin diverses utilisations de ce procédé ainsi que les polypeptides identifiés, y compris leur utilisation dans des trousses de titrage devant permettre de découvrir, modifier et raffiner des médicaments.

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