Polypeptides possessing a nitrilase activity, dna sequence...

Details Polypeptides possessing a nitrilase activity, dna sequence... Polypeptides possessing a nitrilase activity, dna sequence...

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 1/21 (2006.01) C12N 9/78 (2006.01) C12N 15/74 (2006.01) C12P 7/44 (2006.01)

Patent

CA 2103616

IN THE CANADIAN PATENT AND TRADEMARK OFFICE PATENT APPLICATION entitled: Polypeptides possessing a nitrilase activity, DNA sequence coding for said polypeptides, expression cassettes and host microorganisms enabling them to be obtained, and method of converting nitriles to carboxylates by means of said polypeptides in the names of: Dominique PETRE Edith CERBELAUD Sophie LEVY-SCHIL Joël CROUZET Assignee: RHONE POULENC CHIMIE ABSTRACT OF THE DISCLOSURE The present invention relates to novel polypep- tides having a nitrilase activity and to the genetic tools for producing them, namely: - the DNA sequence coding for a polypeptide having a nitrilase activity and capable of hydrolyzing nit- riles to carboxylates, - an analog of this sequence resulting from the degene- racy of the genetic code, - a DNA sequence hybridizing with one of these sequen- ces or a fragment thereof and coding for a poly- peptide having a nitrilase activity, and - expression cassettes and microorganisms enabling them to be obtained. Application: enzymatic conversion of nitriles to carboxylates.

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