Polypeptides with a dopaminergic receptor activity; nucleic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07K 14/705 (2006.01) C07K 16/28 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2060325

The invention relates to new polypeptides with a dopaminergic receptor activity which contain the sequence of 446 amino acids of Figure 1 or a fragment of this sequence, this fragment being such that it nevertheless contains the sites contained in this sequence and whose presence is necessary so that, when this fragment is exposed on the surface of a cell, it is capable of binding the dopamine to its agonists or antagonists in a specific and measurable manner, for example by means of a radioactive ligand; or it is capable of being recognized by antibodies which also recognize the above-mentioned sequence of 446 amino acids but do not recognize either the dopaminergic receptor D-1 or the dopaminergic receptor D-2; or it is capable of generating antibodies which recognize said sequence of 446 amino acids but do not recognize either the dopaminergic receptor D-1 or the dopaminergic receptor D-2.

L'invention a pour objet de nouveaux polypeptides ayant une activité de récepteur dopaminergique contenant: la séquence de 446 acides aminés de la Figure 1, ou un fragment de cette séquence, ce fragment étant tel que, soit il en contient néanmoins les sites contenus dans cette séquence et dont la présence est nécessaire pour que, lorsque ce fragment est exposé à la surface d'une cellule, il soit capable de lier la dopamine a ses agonistes ou antagonistes de que de manière spécifique et mesurable, par exemple au moyen d'un ligand radioactif, soit il est susceptible d'être reconnu par des anticorps qui reconnaissent également la susdite séquence de 446 acides aminés, mais ne reconnaissent ni le récepteur dopamineregique D-1, ni le récepteur dopaminergique D-2, soit il est susceptible de générer des anticorps reconnaissant la susdite séquence de 446 acides aminés mais ne reconnaissant ni le récepteur dopaminergique D-1, ni le récepteur dopaminergique D-2.

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