C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/24 (2006.01)
Patent
CA 2747223
Polypeptides with xylanase activity modified to increase bran solubilisation and/or xylanase activity. The modification comprises at least an amino acid modification i position 110 and may have further modifications of one or more amino acids in position 11, 12, 13, 34, 54, 77, 81, 99, 104, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166 or 175 wherein the positions are determined as the position corresponding the position of Bacillus subtilis xyianase (SEQ ID NO 1). The polypeptides have at least 88% identity with SEQ ID NO 1 or 75% identity to a sequence selected from SEQ ID NO 2-22.
L'invention concerne des polypeptides dotés d'une activité xylanase modifiés pour augmenter la solubilisation du son et/ou l'activité xylanase. La modification comprend au moins une modification d'acide aminé en position 110 et peut comprendre en outre les modifications d'un ou plusieurs acides aminés en position 11, 12, 13, 34, 54, 77, 81, 99, 104, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166 ou 175, les positions étant déterminées comme des positions correspondant aux positions dans la xylanase de Bacillus subtilis (SEQ ID NO 1). Les polypeptides ont une identité d'au moins 88 % avec SEQ ID NO 1 ou une identité de 75 % avec une séquence choisie parmi SEQ ID NO 2-22.
Sibbesen Ole
Sorensen Jens Frisbaek
Danisco A/s
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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