Polysaccharide-protein conjugates reversibly coupled via...

A - Human Necessities – 61 – K

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A61K 47/48 (2006.01) A61K 38/17 (2006.01) A61K 38/28 (2006.01) A61K 47/36 (2006.01) C07K 1/04 (2006.01)

Patent

CA 2728000

A method of preparing an oxidised polysaccharide-protein conjugate by oxidising a polysaccharide with an oxidising agent to form an oxidised polysaccharide and combining such oxidized polysaccharide with a protein. The oxidised polysaccharide is reacted with a protein to form a composition comprising a conjugate wherein the oxidised polysaccharide and the protein are conjugated via one or more imine bonds and wherein the oxidised polysaccharide comprises essentially no alpha-hydroxy aldehyde units. The conjugate may be used to provide sustained or latent activity of the protein.

Linvention concerne un procédé de préparation dun conjugué polysaccharide oxydé-protéine par oxydation dun polysaccharide avec un oxydant pour former un polysaccharide oxydé et combinaison dun tel polysaccharide oxydé avec une protéine. Le polysaccharide oxydé est mis en réaction avec une protéine pour former une composition comprenant un conjugué, le polysaccharide oxydé et la protéine étant conjugués via une ou plusieurs liaisons imine et le polysaccharide oxydé ne comprenant essentiellement aucune unité alpha-hydroxy aldéhyde. Le conjugué peut être utilisé pour fournir une activité prolongée ou latente de la protéine.

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