Polysaccharide ps-a obtained from plants of genus epimedium...

C - Chemistry – Metallurgy – 08 – B

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C08B 37/00 (2006.01) A61K 31/715 (2006.01) C12P 19/04 (2006.01)

Patent

CA 1188687

ABSTRACT OF THE DISCLOSURE A polysaccharide PS-A obtained from plants belonging to the genus Epimedium sp. of the family Berberidaceae and having the following physicochemical properties, a process for preparation thereof and use as phylactic and immuno- stimulating agents comprising the polysaccharide PS-A as an effective component: 1. Elementary analysis: C=40.92, H=6.17, Ash=very small 2. Molecular weight: 75,000+25,000 (average molecular weight) 3. Decomposition point: 205°C 4. pH: 7.0 (solution of 100 mg of PS-A in 50 ml of water) 5. Specific rotatory power: [x]19=-23.6° (in H2O, c=0.527) 6. Infrared absorption spectrum: KBr(cm-1)/3400, 2900, 1620, 1400 max 1230, 1060. 7. Ultraviolet absorption spectrum: maximum absorption is not recognized in the range of 240-400 nm. 8. Outward form: White or faint brown, amorphous powder 9. Solubility: (a) Soluble in water. (b) Insoluble in methanol, ethanol, acetone, ethyl acetate, diethyl ether, hexane or chloroform. 10. Color reactions: Positive to the following reactions: (a) anthrone-sulfuric acid reaction, (b) Molisch's reaction, (c) skatol reaction and (d) Bial's reaction. Negative to the following reactions: (a) ninhydrin reaction, (b) 2,4-DNP reaction, - i - (c) Selivanoff's reaction, (d) naphthoresorcinol reaction and (e) carbazol-sulfuric acid reaction 11. Component sugers: Arabinose and galactose 12. Homogeneity: Homogeneity is proved according to the Ultracentri- fugation, electrophoresis and gel filtration. - ii -

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