Polysaccharide structure and sequence determination

Details Polysaccharide structure and sequence determination Polysaccharide structure and sequence determination

G - Physics

01

N

G01N 33/53 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2373119

The invention provides a method for the structural analysis of a saccharide, comprising: a) providing on a surface a plurality of essentially sequence- and/or site-specific binding agents; b) contacting said surface with a saccharide to be analyzed, or with a mixture comprising a plurality of fragments of said saccharide; c) washing or otherwise removing unbound saccharide or saccharide fragments; d) adding to the surface obtained in step c) an essentially sequence- and/or site-specific marker, or a mixture of essentially sequence- and/or site-specific markers; e) acquiring one or more images of the markers that are bound to said surface; and f) deriving information related to the identity of the saccharide being analyzed from said image.

Cette invention concerne un procédé d'analyse structurelle d'un saccharide, consistant à: a) disposer sur une surface une pluralité d'agents de liaison essentiellement spécifiques de la séquence et/ou région; b) mettre ladite surface en contact le saccharide à analyser, ou un mélange qui renferme plusieurs fragments dudit saccharide; c) retirer, notamment par lavage, le saccharide non lié ou des fragments de saccharide; d) ajouter à la surface obtenue à l'opération c) des marqueurs essentiellement spécifiques de la séquence et/ou région; e) réaliser une ou plusieurs images des marqueurs liés à ladite surface; et f) déduire de ces images des informations sur l'identité du saccharide analysé.

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