Population scale hla-typing and uses thereof

Details Population scale hla-typing and uses thereof Population scale hla-typing and uses thereof

C - Chemistry, Metallurgy

40

B

C40B 40/06 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01)

Patent

CA 2643771

The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.

La présente invention concerne un système portable de génotypage HLA et/ou allélotypage à l'échelle de la population et en temps réel sur le terrain, ainsi que des procédés de génotypage HLA à l'échelle de la population. Les composants individuels du système sont portables et capables de fonctionner sur le terrain, permettant ainsi un rendement élevé en géno- ou allélotypage en temps réel. L'invention concerne également des amorces HLA spécifiques à des gènes et des sondes d'hybridation spécifiques à un polymorphisme mononucléotidique ou à un allèle HLA. La présente invention concerne également un microréseau comprenant les sondes d'hybridation. L'invention concerne également un kit comprenant les amorces HLA spécifiques à des gènes et le microréseau.

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