Portable temperature-sensitive control cassette

Details Portable temperature-sensitive control cassette Portable temperature-sensitive control cassette

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/00 (2006.01)

Patent

CA 1242981

Abstract of the Disclosure Two types of convenient portable control cassettes for the expression of protein encoding sequences in procaryotes are disclosed. Both cassettes comprise the PL promoter from lambda phage, which is controllable by means of a temperature sensitive repressor, operably linked to the ribosome binding site for N-gene (NRBS). In one embodiment, this cassette is bordered by restriction sites upstream of the PL promoter and immediately downstream from the NRBS permitting the insertion of a desired sequence containing its own start codon downstream from the cassette. The other embodiment contains an ATG start codon within the cassette and has a restriction site immediately 3' of the start codon.

473793

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