C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/02 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2456115
The invention encompasses an improved method for measuring membrane potential using compounds of the formula I as petentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPRTR, fluorescent imaging plate reader, sold by Molecular Device Corp., of Sunnyvale, CA; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cells. X is O. or S. n is 1 or 2.
Cette invention concerne une méthode améliorée pour la mesure du potentiel de membrane qui fait intervenir des composés représentés par la formule I comme sondes potentiométriques. Ces sondes peuvent s'utiliser avec d'autres indicateurs fluorescents tels que Indo-1, Fura-2, et Fluo-3, dans des dispositifs de lecture pour plaques de microtitration tels que FLIPR?TR¿, lecteur de plaques imageuses à fluorescence vendu par Molecular Device Corp., de Sunnyvale, CA; des cytomètres de flux et des fluorimètres. Elles servent à mesurer le potentiel de membrane de cellules vivantes. X vaut O. ou S. n vaut 1 ou 2.
Diwu Zhenjun
Kirk Martin
Klaubert Dieter
Yi Guoliang
Mbm Intellectual Property Law Llp
Molecular Devices Inc.
Molecular Devices Corporation
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