Pp32: a newly identified cd45-associated protein

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2182310

This invention relates to a newly identified protein useful for treating diseases of the immune system, methods for obtaining said protein, isolated nucleic acids encoding said protein, and methods for obtaining inhibitors of said protein. The protein of this invention is characterized by an apparent molecular weight of about 32kD, an isoelectric point of about 4.0-4.5 and coprecipitation with CD45. The protein may also be used in in vitro or in vivo assays to identify inhibitors of T cell activation. The invention provides nucleic acid encoding a human 32 kD CD45-associated protein (pp32), recombinant expression vectors and host cells for expressing recombinant forms of human pp32 and a recombinant human pp32 protein free of other human proteins.

La présente invention concerne une protéine récemment identifiée, qui sert au traitement de maladies du système immunitaire, les méthodes permettant d'obtenir ladite protéine, des acides nucléiques isolés codant ladite codéine, et des procédés permettant d'obtenir des inhibiteurs de ladite protéine. La protéine selon l'invention est caractérisée par une masse moléculaire apparente d'environ 32 kD, un point isolélectrique d'environ 4-4, 5 et une coprécipitation avec CD-45. Cette protéine peut aussi s'utiliser dans des dosages in vivo ou vitro pour identifier les inhibiteurs de l'activation des lymphocytes T. L'invention porte sur un acide nucléique codant pour une protéine de 32 kD(pp32), associée à CD-45 des vecteurs d'expression recombinés et des cellules-hôtes pour exprimer des formes recombinées de la protéine humaine pp32 et une protéine pp32 humaine recombinée, exempte d'autres protéines humaines.

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