C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01) C12Q 1/34 (2006.01)
Patent
CA 2490625
An improved method for testing for the presence of a particular microorganism and/or group of microorganisms characterized by a particular enzyme is provided. The method uses a dye-forming and/or fluorogenic-generating substrate throughout a polymer matrix or on the surface of a solid support member that forms a colored precipitate or fluorescent by-product when cleaved by the enzyme. The precipitate is concentrated on the polymer matrix and/or solid support member to create a visible reaction product, Whereas the fluorescent by product will normally be dispersed into the surrounding medium to some extent. The amount of dye-forming substrate needed is independent of the sample size. The present invention further comprises an enzyme indicatordevice comprising a dye-forming and/or fluorogenic-generating substrate throughout a polymer system or on the surface of a solid support member.
Desrosier John P.
Ford Judith
Mallinak Nancy J. S.
Marshall Elliott D. III
Ward N. Robert Jr.
Biocontrol Systems Incorporated
Desrosier John P.
Ford Judith
Gowling Lafleur Henderson Llp
Mallinak Nancy J. S.
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