Precision and accuracy of anion-exchange separation of...

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B01D 15/36 (2006.01) B01J 41/20 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 27/447 (2006.01) G01N 30/34 (2006.01) G01N 30/96 (2006.01) C07H 1/08 (2006.01) G01N 30/02 (2006.01) G01N 30/88 (2006.01)

Patent

CA 2063855

Solvents for salt-gradient anion-exchange separation of nucleic acids, especially double-stranded DNA and especially by liquid chromatography, are improved by replacing NaCl as the eluting salt with any of a wide range of alkyl ammonium salts and can be used to elute nucleic acids in strict order of increasing length, with reduced sensitivity to elution temperature and salt concentration. Anion-exchange chromatography with these solvents is well suited for identification of DNA fragments on the basis of size, with greater accuracy, precision, and resolvable size range than often is possible with gel electrophoresis.

es solvants pour la séparation des acides nucléiques par échange d'anions dans un gradient salin, surtout de l'ADN bicaténaire et surtout par chromatographie en phase liquide, sont améliorés en remplaçant le NaCl comme sel d'élution par un parmi une gamme étendue de sels d'alkyl-ammonium et peuvent être utilisés pour éluer les acides nucléiques strictement par ordre croissant de longueur, tout en réduisant la sensibilité à l'égard de la température d'élution et de la concentration du sel. La chromatographie par échange d'anions avec ces solvants convient bien à l'identification des fragments d'ADN en fonction de leur taille; dans nombre de cas, elle permet une exactitude, une précision et une gamme de tailles séparables plus grandes que l'électrophorèse sur gel.

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