C - Chemistry – Metallurgy – 40 – B
Patent
C - Chemistry, Metallurgy
40
B
C40B 70/00 (2006.01) C12Q 1/68 (2006.01) C40B 20/04 (2006.01) C40B 30/04 (2006.01) D06P 1/00 (2006.01) D06P 1/90 (2006.01) D06P 1/92 (2006.01) G01N 21/64 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2306501
An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.
L'invention concerne un procédé amélioré qui permet de fabriquer une série de populations de billes, de microsphères ou de particules, caractérisées par une variation subtile de la proportion ou du rapport d'au moins deux colorants fluorescents distribués à l'intérieur d'une seule bille de chaque population. Ces billes, quand elles sont excitées par une source de lumière unique, sont capables d'émettre simultanément plusieurs signaux fluorescents. L'invention concerne également un jeu constitué de 64 populations distinctes de billes fluorescentes multicolores qui, combiné à des réactifs analytiques fixés à la surface desdites billes, est extrêmement utile pour l'analyse simultanée d'une pluralité d'analytes dans un seul échantillon. L'invention concerne donc aussi des procédés permettant de colorer des particules polymères et les particules elles-mêmes, ainsi que des méthodes permettant de les utiliser.
Chandler Don
Chandler Van
Lambert Beth
Phipps Stacie
Reber Janet
Luminex Corporation
Robic
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