Preparation and isolation of single-stranded, biotinylated...

Details Preparation and isolation of single-stranded, biotinylated... Preparation and isolation of single-stranded, biotinylated...

C - Chemistry, Metallurgy

07

H

195/1.12, 150/8.

C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2039222

-0- PREPARATION AND ISOLATION OF SINGLE-STRANDED, BIOTINYLATED NUCLEIC ACIDS BY AVIDIN-BIOTIN CLEAVAGE Abstract of the Disclosure A biotinylated target nucleic acid can be isolated from a mixture of nucleic acids using a capture reagent comprising avidin. The targeted nucleic acid is complexed with avidin on a substrate and separated from uncomplexed materials. The complex is heated at above about 65°C for a suitable time to cleave the avidin-biotin bonds to release the desired targeted nucleic acid. Upon release, the targeted nucleic acid can be collected or detected in a suitable manner, such as with a complementary probe. This method is also useful for preparing single-stranded DNA for a number of uses.

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