C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/13 (2006.01) C07K 7/06 (2006.01) C07K 16/00 (2006.01) C07K 16/44 (2006.01) C12N 9/88 (2006.01) C12N 15/10 (2006.01) C12N 15/65 (2006.01) C12N 15/70 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2035381
The invention relates to the preparation and use of gene banks of human antibodies (Ab). Starting from a mixture of human B-lymphocytes, their mRNA is translated into the cDNA using oligo-dT primers. Subsequently, an amplification of the Ab-specific cDNA by means of polymerase chain reaction (PCR) takes place using suitable oligonucleotide primer sequences. Expression of this amplified Ab-specific cDNA in a bacterial expression vector, e.g. the vector pFMT described below, in E. coli thus makes available a human-antibody library with a comprehensive repertoire for screening selected antigens in vitro.
Breitling Frank Berthold
Dubel Stefan
Klewinghaus Iris
Little Melvyn
Seehaus Thomas
Behringwerke Aktiengesellschaft
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Siemens Healthcare Diagnostics Products Gmbh
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