C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/44 (2006.01) A61K 39/145 (2006.01) A61K 39/42 (2006.01) C07K 14/08 (2006.01) C07K 14/085 (2006.01) C07K 16/10 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) G01N 33/569 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2299677
The present invention provides a method of increasing the recombinant expression and solubility of influenza A virus M2 polypeptide comprising nucleic acids encoding a modified M2 protein of influenza A virus in which transmembrane and other hydrophobic domains have been deleted. The present invention also provides purified polypeptides encoded by the nucleic acids, which polypeptides are immunogenic and are less hydrophobic than full-length M2. Also provided are vaccines comprising variants of M2 expressed in prokaryotic hosts. Further provided are methods of preventing influenza A infection using vaccines comprised of variants of M2. Also provided are antibodies raised against the variants of M2, and use of such antibodies in diagnosis and treatment of influenza A infections.
L'invention concerne une technique qui permet d'augmenter l'expression recombinante et la solubilité du polypeptide M2 du virus grippal A, ledit virus renfermant des acides nucléiques qui codent pour une protéine M2 modifiée dans laquelle des domaines transmembranaires et d'autres domaines hydrophobes ont été délétés; des polypeptides purifiés codés par lesdits acides nucléiques, qui sont immunogènes et moins hydrophobes que la protéine M2 pleine longueur; des vaccins renfermant des variants de M2 exprimés dans des cellules procaryotes; des techniques qui permettent de prévenir l'infection par le virus grippal A au moyen de vaccins renfermant des variants de M2; et des anticorps produits contre les variants de M2, ainsi que leur utilisation dans le diagnostic et le traitement des infections par le virus grippal A.
Frace A. Michael
Katz Jacqueline M.
Klimov Alexander I.
C/o Centers For The Government Of The United States Of America As Represented By
Ridout & Maybee Llp
LandOfFree
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