Preparation exhibiting enzymatic delignification activity, a...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/24 (2006.01) A61K 39/395 (2006.01) C07H 21/00 (2006.01) C07K 16/40 (2006.01) C12N 1/20 (2006.01) C12S 3/08 (2006.01) D21C 5/00 (2006.01) D21C 9/00 (2006.01)

Patent

CA 2073436

2073436 9110724 PCTABS00006 A preparation exhibiting enzymatic activity and having the capability of delignifying wood pulp at a temperature of at least 65 ·C and a pH of at least 9, is described. Said preparation is obtainable by aerobic fermentation of a Bacillus stearothermophilus strain selected from the deposited strains NCIMB 40221 and NCIMB 40222 and mutants and variants thereof. Also, the deposited strains NCIMB 40221 and NCIMB 40222 and mutants and variants thereof are comprised by the invention. Further, a method of producing said preparation and a process comprising treatment of wood pulp with said preparation, are disclosed. Moreover, the invention comprises applications of said preparation as wood pulp and fluff pulp treated with said preparation, and paper, board and fluff made from a wood pulp treated with said preparation. Additionally, DNA and RNA probes which recognize the nucleotide sequence coding for a specified amino acid sequence, and also an antibody which binds to said amino acid sequence are included.

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