A - Human Necessities – 01 – N
Patent
A - Human Necessities
01
N
47/2, 71/4.5
A01N 63/00 (2006.01) A01C 1/08 (2006.01) A01N 63/04 (2006.01)
Patent
CA 1133270
ABSTRACT OF THE DISCLOSURE The invention relates to a dry powdered preparation to be applied onto sugar beet seeds, the preparation containing, as its active consti??? organs of reproduction of Pythium oligandrum Drechsler, particularly the oospores, in a sufficient- ly high concentration of at least about 1,000,000, preferably at least about 4,000,000 per gram (most preferably in the range of 50,000,000 - 500,000,000 per gram). Such concentrations enable to apply more than 100,000 oospores or more onto every seed, which proved sufficient to secure protection of emerging suger beet plants against "damping off" or "black leg" disease. Although the mycoparasitism of Pythium oligandrum has been dis- covered many years ago, its use for biological protection re- quired a lot of research work establishing the conditions necessary for obtaining reliable results on a large scale. Crucial conditions are: a) dry powdered preparation, and b) an unusually high concentration of oospores, namely at least about 1,000,000, (preferably in the range 50,000,000 - 500,000,000 per gram) measured in a known way using a B?rker's chamber. The most suitable method for producing such powdered preparation consists in cultivating Pythium oligandrum in stationary cul- tures on grain or corn substrate wetted with liquid nutrients containing, in addition to usual constituents such as sugars, corn-steep liquor, and mineral salts, from 2 to 3 percent of calcium chloride. The sterilized substrate is then inoculated with Pythium oligandrum Drechsler and the cultivation is carried out preferably under semi-sterile conditions, the substrate being irradiated with visible light, advantageously through a blue or green filter. Another feasible method consists in submerse cultivation of P. oligandrum and subsequent dehydration of the fermented mash either by adding substances capable of binding water such as sugars, starch or mineral salts, or by lyophilization, or by spray-drying. In any case the substance is dried and ground at temperatures not exceeding 35°C. The powdered preparation thus obtained is ready for use and can be stored for several months without losing mast of its activity.
331172
Hejdanek Slavomil
Vesely Dasa
Swabey Ogilvy Renault
Vyzkumny Ustav Rostlinne Vyroby
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