Preparation of autologous haematopoietic stem cells, the...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01) A61K 35/14 (2006.01) A61P 25/00 (2006.01) A61P 25/28 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2601679

The invention applies to the field of cell transplantology and neurology and can be used to treat wound dystrophy of the central nervous system. The main idea of the invention is a preparation of haematopoietic stem cells (HSC), which is autologous HSC extracted from patient's peripheral blood enriched with cells containing CD34 antigen, in the final concentration of (40-100)x106 cells/ml. Enriching the blood is performed using intermittent administration of the preparation of the granulocyte colony-stimulating factor (G-CSF) - for example, Neilogen in the glucose solution, according to a certain scheme. Also, the method of production, cryoconservation of the preparation and the method of treating traumatic diseases in the brain and spinal cord, using the new preparation. The developed system allows patients to be helped with neurological diseases efficiently and suffering minimum injuries.

L'invention se rapporte au domaine de la transplantologie et de la neurologie cellulaires, et peut servir à traiter une dystrophie du système nerveux central liée à une plaie. Cette invention concerne principalement une préparation de cellules souches hématopoïétiques (HSC) de type autologues qui sont extraites du sang périphérique d'un patient, enrichi de cellules contenant l'antigène CD34 à une concentration finale de (40-100)x106 cellules/ml. L'enrichissement du sang se fait par administration intermittente d'une préparation du facteur stimulant les colonies de granulocytes (G-CSF), par exemple le produit Neupogen dans une solution de glucose, selon un schéma donné. Cette invention concerne également un procédé de production et de cryoconservation de ladite préparation, et un procédé pour traiter des maladies traumatiques affectant le cerveau et la moelle épinière, au moyen de cette préparation. L'invention permet d'aider des patients présentant des maladies neurologiques, d'une manière efficace et de façon à entraîner le moins de lésions possible.

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