Preparation of cartilage extracts using organic solvents

A - Human Necessities – 61 – K

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A61K 35/60 (2006.01) A61K 35/32 (2006.01)

Patent

CA 2435586

This invention relates to a process by which organic solvent-containing solutions are used in lieu of pure water for the preparation of cartilage extracts and fractions thereof. Different organic solvents have been tested for the preparation of extracts containing biologically active components. Amongst the tested solvents, trimethylamine 40% (in water) was selected as a good alternative solvent to pure water, particularly in recovering an anti- proliferative activity against HUVECs.

L'invention concerne un procédé consistant à utiliser des solutions contenant des solvants organiques à la place d'eau pure afin de préparer des extraits de cartilage et des fractions de ces derniers. Différents solvants organiques ont été testés pour la préparation d'extraits contenant des composants biologiquement actifs. Parmi les solvants testés, la triméthylamine à 40% (dans l'eau) a été sélectionnée comme bonne alternative à l'eau pure, en particulier pour récupérer une activité antiproliférative contre des HUVEC.

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