Preparation of cellular components that are substantially...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) A61K 31/711 (2006.01) C12N 1/21 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2386401

The invention relates to a method of purifying a cellular component substantially free of chromosomal DNA. The method comprises culturing cells producing the cellular component in a medium. The chromosomal DNA of the cells is then exposed to a restriction enzyme such that it is cleaved. The cellular component to be purified is resistant to such cleavage.

L'invention concerne un procédé de purification d'un composant cellulaire, sensiblement dépourvu d'ADN chromosomique. Ce procédé consiste à cultiver, dans un milieu, des cellules produisant le composant cellulaire, puis à exposer l'ADN chromosomique des cellules à une enzyme de restriction de manière que cet ADN soit clivé, le composant cellulaire à purifier étant résistant à un tel clivage.

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