Preparation of deallergenized proteins and permuteins

Details Preparation of deallergenized proteins and permuteins Preparation of deallergenized proteins and permuteins

C - Chemistry, Metallurgy

12

N

C12N 15/29 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A61K 39/35 (2006.01) C07K 14/415 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 5/14 (2006.01) C12N 9/14 (2006.01) C12N 9/18 (2006.01) C12N 15/00 (2006.01) C12N 15/82 (2006.01) A01N 65/00 (2006.01)

Patent

CA 2396422

Modified proteins are disclosed that maintain enzymatic and insecticidal activity while displaying reduced or eliminated allergenicity. Epitopes which bind to anti-patatin antibodies were identified, and removed via site directed mutagenesis. Tyrosines were observed to generally contribute to the allergenic properties of patatin proteins. Removal of glycosylation sites was observed to reduce or eliminate antibody binding. Permuteins are also disclosed which have a rearranged amino acid sequence while retaining enzymatic activity. Deallerginized proteins and permuteins can be used as insecticidal materials, as nutritional supplements, and as immunotherapeutic agents.

L'invention concerne des protéines modifiées qui conservent une activité enzymatique et insecticide tout en affichant une allergénicité réduite ou supprimée. Des épitopes se liant à des anticorps anti-patatine sont identifiés et éliminés via une mutagenèse orientée site. On a observé que les tyrosines contribuent généralement aux propriétés allergéniques des protéines de patatine. On a également observé que la suppression de sites de glycosylation réduit et supprime une liaison d'anticorps. L'invention concerne également des permuprotéines possédant une séquence d'acide aminé réagencée tout en conservant une activité enzymatique. On peut utiliser les protéines et les permutéines désallergénisées comme matériaux insecticides, compléments alimentaires, et agents immunothérapeuthiques.

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