Preparation of enzymes having altered activity

C - Chemistry – Metallurgy – 12 – N

Patent

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195/44, 195/33.1

C12N 9/14 (2006.01) C12N 9/16 (2006.01) C12N 9/20 (2006.01) C12N 9/50 (2006.01) C12P 7/64 (2006.01)

Patent

CA 1341421

The invention relates to altering the transesterification rate/hydrolysis rate ratio and nucleophile specificity in reactions that are catalyzed by enzymes with catalytic triads such as serine hydrolases and cystine hydrolases by using a novel method to modify the enzyme. Modifications to the enzyme are achieved by altering amino acid residues within 15 angstroms of the catalytic triad in the enzyme.

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