C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/44, 195/33.1
C12N 9/14 (2006.01) C12N 9/16 (2006.01) C12N 9/20 (2006.01) C12N 9/50 (2006.01) C12P 7/64 (2006.01)
Patent
CA 1341421
The invention relates to altering the transesterification rate/hydrolysis rate ratio and nucleophile specificity in reactions that are catalyzed by enzymes with catalytic triads such as serine hydrolases and cystine hydrolases by using a novel method to modify the enzyme. Modifications to the enzyme are achieved by altering amino acid residues within 15 angstroms of the catalytic triad in the enzyme.
546300
Arbige Michael V.
Estell David A.
Pepsin Michael J.
Poulose Ayrookaran J.
Genencor Inc.
Sim & Mcburney
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