Preparation of highly-purified plasma membranes

Details Preparation of highly-purified plasma membranes Preparation of highly-purified plasma membranes

G - Physics

01

N

G01N 33/543 (2006.01) B03C 1/00 (2006.01) C07K 16/28 (2006.01) C07K 16/30 (2006.01) C12Q 1/00 (2006.01) C12Q 1/24 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/553 (2006.01) G01N 33/554 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2460568

The invention features a method for purifying a cell surface plasma membrane, the method including the steps of: (a) contacting a biological sample with an antibody or antigen-binding fragment thereof that specifically binds to a plasma membrane entigen, thereby forming an antibody/plasma membrane complex; and (b) recovering the antibody/plasma membrane complex, thereby purifying the plasma membrane from the biological sample. The invention further features methods for identifying plasma membrane polypeptides using mass spectrometry and purified plasma membrane fractions.

L'invention porte sur un procédé de purification de la membrane plasmique de la surface de cellules comportant les étapes suivantes: (a) mise en contact d'un échantillon biologique avec un anticorps ou l'un de ses fragments de fixation se fixant spécifiquement à un antigène de la membrane plasmique de manière à former un complexe anticorps/membrane plasmique, et (b) récupération dudit complexe ayant pour effet de purifier la membrane plasmique de l'échantillon biologique. L'invention porte également sur des procédés d'identification par spectroscopie de masse de polypeptides de membrane plasmique, et sur des fractions purifiées de membrane plasmique.

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