G - Physics – 01 – N
Patent
G - Physics
01
N
402/3, 167/44
G01N 33/532 (2006.01) G01N 33/531 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 33/546 (2006.01) G01N 33/72 (2006.01)
Patent
CA 1305809
ABSTRACT OF THE DISCLOSURE A method for chemically coupling a control- lable number of ligands, e.g., haptens, to a poly- meric material by derivatization of the polymer with an activating agent to introduce a couplable functional group. The derivatization is performed in the presence of a blocking agent which is reactive with the same functionality on the polymer as the activating agent. By reacting the polymer with a mixture of a predetermined ratio of excess amounts of the activating and blocking agents, a controllable number of couplable functional groups are introduced to the polymer for subsequent linkage to the desired ligand. The resulting polymer-ligand conjugates are useful as reagents in immunoassays, particularly immunoturbidimetric assays. MS-1510
576687
Lewis Lynette A.
Yip Kin Fai
Miles Inc.
Osler Hoskin & Harcourt Llp
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