Preparation of monoclonal antibodies capable of reacting...

Details Preparation of monoclonal antibodies capable of reacting... Preparation of monoclonal antibodies capable of reacting...

C - Chemistry, Metallurgy

12

P

195/1.109

C12P 21/08 (2006.01) C07K 16/30 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1315220

The object of the present invention is the production of monoclonal antibodies (MCAs) that react with a high degree of selectivity to human breast cancer cells for a potential use in tumor diagnosis,prognosis and therapy. MCAs are produced by fusing Ns-l myeloma cells to spleen cells from BALB/c mice hyperimmunized with the well- characterized human breast carcinoma cell line BT-20. Hybridoma are selected on the basis of their preferential binding in an ELISA assay to human mammary tumor cells versus normal breast cells. Ammonium sulphate precipitation and ion-exchange chromatography allow the isolation of partially purified material that is further used as a possible tool to distinguish breast carcinoma cells from normal cells of the same histogenesis. According to the invention, three different techniques are used: ELISA, dot-immunobinding, and indirect immunofluorescence, to further characterize the antigen recognized by each of the MCAs produced.

498684

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