Preparation of nucleic acid from mononuclear cells

Details Preparation of nucleic acid from mononuclear cells Preparation of nucleic acid from mononuclear cells

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2137563

Method and kit for the preparation of nucleic acid, such as for amplification. The nucleic acid may be prepared by centifu- ging a sample of whole cells, particularly mononuclear cells, with an appropriate centrifugation medium so as to separate desired cells from non-desired cells, then removing and lysing said desired cells. A complexing agent able to complex ferric ion may be added to the lysed cells. The nucleic acid associated with the lysed cells can be amplified, and the nucleic acid can also be screened for a certain nucleic acid sequence, such as a viral nucleic acid sequence.

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