Preparation of peptides with c-terminal proline amide

Details Preparation of peptides with c-terminal proline amide Preparation of peptides with c-terminal proline amide

C - Chemistry, Metallurgy

12

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195/134.7

C12P 21/02 (2006.01) C07K 1/00 (2006.01) C07K 14/585 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1251153

ABSTRACT OF THE DISCLOSURE A peptide with C-terminal proline amide is produced by reacting in aqueous solution a protein substrate having C-terminal prolyl-leucine, prolyl-isoleucine, prolyl-valine, or prolyl-phenylalanine with carboxypeptidase Y in the presence of ammonia. The ammonia is preferably generated by the aqueous reaction of an ammonium salt and an alkali. An example of the peptide product is human calcitonin.

506086

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