Preparation of polypeptides

Details Preparation of polypeptides Preparation of polypeptides

C - Chemistry, Metallurgy

12

P

195/1.22, 195/1.

C12P 21/02 (2006.01)

Patent

CA 1302321

38 ABSTRACT OF THE DISCLOSURE A polypeptide comprising the A and B polypeptide chains of human insulin is prepared. A microorganism transformed by an expression transfer vector comprising a deoxynucleotide sequence coding for human proinsulin is incubated under conditions suitable for expression of the sequence coding for human proinsulin, and the human proinsulin is purified from the lysate or culture medium of the microorganism.

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