C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/129, 530/7.1
C12N 15/00 (2006.01) C07H 21/00 (2006.01) C07K 14/60 (2006.01) C12P 21/02 (2006.01)
Patent
CA 1224166
Abstract of the disclosure: Polypeptides having a carboxamide carboxyl ter- minal end and a methionine residue, which is optionally bonded to a bacterial protein, on the amino end can be prepared by synthesis by genetic engineering methods of the corresponding polypeptide having at the C terminal end a glycine residue, and conversion of the latter enzymatic- ally into the amino group of the desired carboxamide group. Peptides which have the aminoacid sequence of growth hormone releasing factor, part sequences thereof, or modifications of these peptides, are readily accessible by this means. The synthesis by genetic engineering methods is advantageously carried out via two gene frag- ments which are synthesized chemically from smaller, single stranded structural units. The two gene fragments are then linked enzymatically to give the complete gene, which is incorporated into a suitable vector, amplified there, and the peptide is isolated directly or as a fused protein, and is converted enzymatically into the desired amide.
459771
Engels Joachim
Konig Wolfgang
Mullner Hubert
Uhlmann Eugen
Wetekam Waldemar
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Hoechst Aktiengesellschaft
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