Preparation of protective antigen from bacillus anthracis

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/31 (2006.01) A61K 39/07 (2006.01) A61P 31/04 (2006.01) C07K 14/32 (2006.01) C12N 1/21 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2570508

A polynucleotide sequence is provided, comprising: a nucleic acid sequence having at least 75 % identity to SEQ ID NO: 1, wherein said nucleic acid sequence encodes recombinant Bacillus anthracis Protective Antigen (rPA); or a fragment of said nucleic acid sequence wherein said fragment encodes a fragment of recombinant Bacillus anthracis Protective Antigen (rPA). Also provided are expression vectors and host cells comprising the polynucleotide sequence of the invention, and methods for producing rPA or a fragment thereof. The invention further provides antigenic compositions and. corresponding methods and uses for inducing an immune response.

L'invention concerne une séquence polynucléotidique comprenant: une séquence d'acide nucléique présentant identique au moins à 75 % à SEQ ID NO: 1. Cette séquence d'acide nucléique code un antigène protecteur recombinant (rPA) de Bacillus anthracis, ou un fragment de la séquence d'acide nucléique, lequel fragment code un fragment de l'antigène protecteur recombinant (rPA) de Bacillus anthracis. L'invention concerne également des vecteurs d'expression et des cellules hôtes comprenant la séquence polynucléotidique de l'invention, et des méthodes de production de rPA ou de son fragment. L'invention concerne en outre des compositions antigéniques et des méthodes et utilisations correspondantes pouvant induire une réponse immunitaire.

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