Preparation of recombinant borrelia proteins

Details Preparation of recombinant borrelia proteins Preparation of recombinant borrelia proteins

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) A61K 39/02 (2006.01) C07K 1/14 (2006.01) C07K 1/16 (2006.01) C07K 14/20 (2006.01) C12N 1/06 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2121121

Highly-purified immunologically-effected recombinant protein encoded by a gene of a full-length wild-type Borrelia lipoprotein, particularly the OspA encoded by the ospA gene of B.burgdorferi, useful in vaccines and test kits for Lyme disease, is prepared by inducing protein production from a host organism transformed by a plasmid vector containing the cloned gene and subsequently recovering and purifying the protein. The grown organism is lysed and contacted with a surfactant, particularly Triton X-114, which selectively extracts the desired protein from the lysed cells. Upon heating the resulting mixture to mildly-elevated temperature, a detergent phase separates out and is recovered separate from the other phases. The detergent phase is further purified from residual protein by column chromatography.

Affiliated with

Also associated with

Rating

Preparation of recombinant borrelia proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Preparation of recombinant borrelia proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Preparation of recombinant borrelia proteins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1854789