Preparation of recombinant factor viii in a protein free medium

Details Preparation of recombinant factor viii in a protein free medium Preparation of recombinant factor viii in a protein free medium

C - Chemistry, Metallurgy

07

K

C07K 14/755 (2006.01) C12P 21/02 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2234215

Recombinant Factor VIII can be produced in relatively large quantities on a continuous basis from mammalian cells in the absence of any animal-derived proteins such as albumin by culturing the cells in a protein free medium supplemented with polyol copolymers, preferably in the presence of trace metals such as copper. In very preferred embodiments, the medium includes a polyglycol known as Pluronic F-68, copper sulfate, ferrous sulfate/EDTA complex, and salts of trace metals such as manganese, molybdenum, silicon, lithium and chromium.

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