Preparation of vaccine master cell lines using recombinant...

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01)

Patent

CA 2628308

The subject invention provides a plant cell culture for producing proteinaceous agents comprising a plant cell line stably transformed to express a transgene encoding a proteinaceous agent and a growth medium which supports the growth of said plant cell culture but which does not support the growth of Mycoplasmataceae and contains no materials of animal origin. The plant cell line is capable of being continuously passaged such that consistent transgene expression is maintained during passaging. The plant cell line is also capable of being cryopreserved such that consistent transgene expression is recovered upon recovery from cryopreservation.

La présente invention concerne une culture de cellules végétales pour produire des agents protéiques comprenant une lignée cellulaires transformée de manière stable pour exprimer un transgène codant un agent protéique et un milieu de développement qui supporte le développement de ladite culture de cellules végétales, mais qui ne supporte pas le développement de Mycoplasmataceae et qui ne contient aucun matériau d'origine animale. La lignée cellulaire végétale peut être passée de façon continue de sorte que l'expression de transgène constante soit préservée au cours du passage. La lignée cellulaire végétale peut également être conservée à une température cryogénique de sorte que l'expression de transgène constante soit recupérée après la récupération à partir de la cryoconservation.

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