Preparations of islet amyloid polypeptide (iapp) and...

C - Chemistry – Metallurgy – 07 – K

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530/7.06, 530/7.

C07K 14/47 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C07K 16/18 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1341222

Islet amyloid polypeptide substantially free of islet amyloid which can be isolated from islet amyloid of different mammals and when isolated from humans it has the following amino acid sequence in positions 1-37: Lys-Cys-Asn-Thr-Ala-Thr-Cys-Ala- Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Val-His-Ser-Ser-Asn-Asn-Phe- Gly-Ala-Ile-Leu-Ser-Ser-Thr-Asn-Val-Gly-Ser-Asn-Thr-Tyr. While other attempts have been made to isolate and characterize the major component of islet amyloid, none have been successful. Proteins containing the amino acid sequence of the polypeptide or of active fragments of the polypeptide may be employed for diagnosis of disease related to the biological function of the polypeptide. The anti-IAPP antibody preparations provided by the present invention can be used to determine IAPP concentration in biological samples.

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