Preparative electrophoresis device and method

G - Physics – 01 – N

Patent

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Details

G01N 27/453 (2006.01) B01D 57/02 (2006.01) C07K 1/26 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2137155

A preparative electrophoresis device (10) having a pair of spaced apart plates (12, 14), a pre-cast electrophoretic gel (26, 28) between the plates, and a semipermeable membrane (30) sealably connected to ends of the plates to define with the plates a collection channel (36) for receiving molecules that have traveled through the gel. Also disclosed are close spacing (less than 2.0 mm) of the plates, an inlet tube (22) and an outlet tube (24) connected to the two plates and communicating with opposite ends of the collection channel, an elongated divider (18) between the plates to divide the gel into a purification region (28) and an indicating region (26) that is not as wide as the purification region and receives marker molecules of known molecular weight to indicate the location in the indicating region of molecules of interest, and pumping electrophoretic buffer into the collection channel at the same rate that it is pumped out.

Le dispositif (10) de préparation par électrophorèse se compose d'une paire de plaques (12, 14) espacées entre lesquelles est prévu un gel électrophorétique (26, 28) précoulé, et d'une membrane (30) semi-perméable raccordée de manière hermétique aux extrémités desdites plaques afin de définir avec celles-ci un canal collecteur (36) destiné à recevoir les molécules ayant traversé le gel. L'espacement serré (moins de 2 mm) des plaques est également décrit. Un tube d'admission (22) et un tube de sortie (24) raccordés aux deux plaques et communiquant avec les extrémités opposées du canal collecteur, une séparation allongée (18) entre les plaques permettant de diviser le gel en une région de purification (28) et une région indicatrice (26) moins large recevant des molécules marqueuses de masse molaire connue indiquant l'emplacement de la région indicatrice des molécules en question, et pompant le tampon électrophorétique dans le canal collecteur au même rythme qu'il est évacué, sont prévus.

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