Preparing process of physiologically active substance with...

A - Human Necessities – 61 – K

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A61K 35/12 (2006.01) C07G 99/00 (2009.01)

Patent

CA 1326996

Abstract: Preparing process of physiologically active substance with anticancer effect and the substance prepared by the process. This process comprises: transplanting of cancer cells in a mammal abdominal cavity; administering an anticancer agent in the abdominal cavity; intravenously administering spleen cells extracted from a normal and the same kind of mammal in a vena of the mammal to which cancerous cells have been transplanted; collecting ascites retained in the abdominal cavity after elapse of a predetermined time period; and fractionating a supernatant liquid of the ascites by Cohn's fractionation to isolate Cohn V fraction of said supernatants.

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