Preservation of rna in a biological sample

Details Preservation of rna in a biological sample Preservation of rna in a biological sample

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C40B 30/04 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2523501

To preserve RNA in a biological sample for analysis, the sample is incubated with an RNA preservative capable of precipitating RNA in an aqueous solution, such as a triphenylmethane dye (e.g., methyl green, crystal violet, pararosaniline, or tris-(4-aminophenyl)methane), cresyl violet, or cobalt ions. RNA preservation may be used in an immunostaining assay and other histochemical methods.

La présente invention a trait à la préservation d'ARN dans un échantillon biologique pour analyse qui comprend l'incubation de l'échantillon avec un agent de préservation capable d'assurer la précipitation de l'ARN dans une solution aqueuse, telle qu'un colorant de triphénylméthane (par exemple, vert de méthyle, violet de cristal, pararosaniline, ou tris-(4-aminophényl)méthane), violet de crésyle, ou des ions de cobalt. La préservation d'ARN peut être utilisée dans une bioanalyse de marquage immunologique ou d'autres procédés histochimiques.

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