Preserved cell preparations for flow cytometry and immunology

Details Preserved cell preparations for flow cytometry and immunology Preserved cell preparations for flow cytometry and immunology

C - Chemistry, Metallurgy

12

N

C12N 5/08 (2006.01) A01N 1/00 (2006.01) C12N 5/06 (2006.01) G01N 33/554 (2006.01) G01N 33/96 (2006.01)

Patent

CA 2147062

This invention discloses a population of cells which have been preserved in non-frozen hydrated form, and which have been chemically treated in a manner that renders them metabolically inert and free of proteolytic enzyme activity, but without significantly altering the cell surface proteins that are of interest in flow cytometry. The absence of alteration of the surface proteins is indicated by the ability of the surface proteins to bind to monoclonal antibodies that bind to untreated proteins of the same type, with approximately the same affinity as proteins on untreated cells. The preserved cells and their surface antigens are stable for at least a month when stored at 4°C in buffered saline solution. These cell preparations are useful as quality control (QC) reagents for processes such as calibrating and standardizing flow cytometry equipment, and for use as "unknown" test samples for QC testing programs, or as patient specimens for archival storage and subsequent retrospective analysis. This invention also discloses methods of carrying out such treatment to preserve cell populations.

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