Pretreatment reagents and methods using the same

G - Physics – 01 – N

Patent

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Details

G01N 33/53 (2006.01) G01N 1/28 (2006.01) G01N 33/68 (2006.01) G01N 33/94 (2006.01) G02B 6/42 (2006.01)

Patent

CA 2230284

Compositions and kits are disclosed for pretreating samples that are to be analyzed for the presence and/or amount of an associated analyte. The composition comprises a lower alkyl alcohol in an amount of about 30 % to about 40 % by volume, a glycol in an amount of about 20 % to about 40 % by volume, and an aqueous component comprising about 20 mM to about 30 mM copper salt. Additionally, the aqueous component can comprise about 0.5 mM to about 20 mM of a buffer, and about 0.005 % to about 0.2 % by weight of a non-ionic detergent and has a pH of about 2.0 to about 4.6. The kits further include one or more reagents for conducting an assay for the associated analyte. Also disclosed are improvements in assays for associated analytes wherein the improvements comprise pretreating a sample suspected of containing the associated analyte with the above composition.

L'invention concerne des compositions et des kits destinés au prétraitement d'échantillons devant être analysés afin de déterminer la présence et/ou la quantité d'une substance à analyser. La composition comprend un alcool d'alkyle inférieur, dans une proportion d'environ 30 à 40 % en volume, un glycol, dans une proportion d'environ 20 à 40 % en volume, et un composant aqueux comprenant environ 20 à 30 mM de sel de cuivre. En outre, le composant aqueux peut comprendre environ 0,5 à 20 mM d'un tampon, et environ 0,005 à 0,2 % en poids d'un détergent non ionique et présente un pH d'environ 2,0 à environ 4,6. Les kits comprennent également un ou plusieurs réactifs destinés à la réalisation d'un dosage pour la substance à analyser associée. L'invention concerne également des améliorations apportées à des dosages destinés à des substances à analyser associées, lesdites améliorations comprenant le prétraitement d'un échantillon supposé contenir la substance à analyser avec la composition ci-dessus.

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